Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum.

Authors

  • L Pinheiro Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Lisboa.
  • V E Rosário

DOI:

https://doi.org/10.20344/amp.1190

Abstract

In 3 different geographical areas of West Africa (Guinea-Bissau, S. Tomé e Princípe and Angola) where chloroquine activity against P. falciparum seems to have decreased, the choice for prevention and/or treatment of malaria is often based on second line drugs such as Fansidar® (pyrimethamine/sulphadoxine). Little is known about the genetic basis of dhfr-ts gene mutations from parasites from these areas. In this study a PCR/RFLP methodology was used to screen 33 field isolates, without intervening steps of in vitro culture, for the pyrimethamine and cycloguanil sensitive/resistant genotype of P. falciparum. Analysis of the digested fragments revealed a prevalence of a Ser108 to Asn108 change in 63.63% of the samples tested.

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How to Cite

1.
Pinheiro L, Rosário VE. Presence of pyrimethamine and cycloguanil resistance genotype in West Africa? Evidence of a single mutation 59Arg in the dhfr gene of Plasmodium falciparum. Acta Med Port [Internet]. 2003 Aug. 31 [cited 2024 Nov. 14];16(4):235-8. Available from: https://actamedicaportuguesa.com/revista/index.php/amp/article/view/1190

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