Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina.

L Pinheiro; S Franco; I S Adagu; R Rosa; V E Rosário; D C Warhurst

doi:10.20344/amp.1191

Presence of the double pfmdr1 mutation 86Tyr and 1246 Tyr in clones of a chloroquine-resistant west African isolate of Plasmodium falciparum.

Authors

L Pinheiro Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Lisboa.
S Franco
I S Adagu
R Rosa
V E Rosário
D C Warhurst

DOI:

https://doi.org/10.20344/amp.1191

Abstract

Isolates of Plasmodium falciparum from three areas of West Africa were recovered from cryopreservation and their chloroquine-sensitivity were determined in vitro. Of the 90 samples studied, 60 were from Guinea-Bissau (30Resistant/30Sensitive), 15 were from S. Tomé and Príncipe (11Resistant/4Sensitive) and 15 were from Angola (11Resistant/4Sensitive). All the isolates were sensitive to mefloquine. Using the polymerase chain reaction/restriction fragment length polymorphism technique (PCR/RFLP) it was possible to detect two mutations in the pfmdr1 gene, often associated with chloroquine-resistance. 66% of the samples from Guiné-Bissau showed a correlation with chloroquine-resistance while 73% of the samples from São Tomé and Angola altogether had the 86Tyr mutation. The present study on West African isolates and clones showed, for the first time, the presence of a double point mutation in the pfmdr1 gene one being found, up to now, only in South America isolates of Plasmodium falciparum.

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How to Cite

Pinheiro L, Franco S, Adagu IS, Rosa R, Rosário VE, Warhurst DC. Presence of the double pfmdr1 mutation 86Tyr and 1246 Tyr in clones of a chloroquine-resistant west African isolate of Plasmodium falciparum. Acta Med Port [Internet]. 2003 Aug. 31 [cited 2024 Nov. 23];16(4):229-33. Available from: https://actamedicaportuguesa.com/revista/index.php/amp/article/view/1191

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Issue

Vol. 16 No. 4 (2003): July-August

Section

Arquivo Histórico

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