Formaldehyde fixation for selective immunofluorescence of membrane antigens.
DOI:
https://doi.org/10.20344/amp.3901Abstract
Evidencia-se, por imunofluorescência, um componente de membrana do antigénio T específico do adenovírus tipo 12 em células transformadas por esse vírus fixadas com formaldeído. A localização do antigénio na membrana celular demonstra-se por prova de ligação de S. aureus. Os componentes intracelulares do antigénio T não se observam nas células fixadas com formaldeído e a impermeabilidade celular garantida por essa fixação demonstra-se pela negatividade de imunofluorescência com o soro anti-actina.
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